File Name: lipid structure and function .zip
Figure 1. Hydrophobic lipids in the fur of aquatic mammals, such as this river otter, protect them from the elements.
Membrane lipids are a group of compounds structurally similar to fats and oils which form the double-layered surface of all cells lipid bilayer. The three major classes of membrane lipids are phospholipids , glycolipids , and cholesterol. Lipids are amphiphilic: they have one end that is soluble in water 'polar' and an ending that is soluble in fat 'nonpolar'. By forming a double layer with the polar ends pointing outwards and the nonpolar ends pointing inwards membrane lipids can form a 'lipid bilayer' which keeps the watery interior of the cell separate from the watery exterior.
Service Unavailable in EU region
Hundreds of different lipid species are present in eukaryotic cell membranes. Some of them aggregate with specific membrane proteins to form specialized domains that concentrate and control cellular trafficking and signaling events. For both naked and enveloped viruses, viral entry, genome replication, and egress involve specific interactions with the membranes of a susceptible host cell.
Their characterization has led to the discovery of lipid-active compounds as potential antiviral drugs. High-resolution mass spectrometry streamlines the shotgun analysis of total lipid extracts. It can distinguish and quantify isobaric lipid species and has potential to become the "gold standard" lipidomics methodology. Questions in modern lipid cell biology have been answered by membrane models such as SUVs, LUVs, and GUVs—closed lipid bilayers that are used to study effects of membrane permeability barriers and curvature.
However, new membrane models are needed for answering pressing questions regarding membrane structure and function in nonequilibrium states. Most of the thousands of different membrane lipids in cells are synthesized by enzymes in the ER. Glycerophospholipid, sphingolipid, and sterol transfer proteins and transport them to the compartments where they reside.
Some viruses and bacterial toxins enter cells by binding to membrane glycosphingolipids. Their binding changes the properties of the membrane, inducing curvature, trans -bilayer coupling, and, ultimately, internalization. PtdIns 3,4,5 P 3 specifies the basolateral surface of polarized epithelial cells, whereas PtdIns 4,5 P 2 specifies the apical domain. Individual cells also use phosphoinositides to further organize into epithelial tissues.
Sterol-regulatory-element-binding proteins SREBPs are cleaved and released from membranes when cells are deprived of cholesterol or fatty acids. They migrate to the nucleus and up-regulate genes required for lipid synthesis and uptake.
More than glycolipids derived from glucosyl or galactosyl ceramide exist in cells. These molecules help define specific domains of cellular membranes, in which they regulate protein trafficking and signal transaction. The temporal dynamics of cellular lipids and membranes can be captured using multiple fluorescence-based microscopy techniques. In conjunction with model membrane systems to study lipid dynamics, these techniques have propelled recent research in lipid cell biology.
Three factors contributing to morphological plasticity in cell membranes include nonbilayer lipids e. Lipids can act as a glue that holds oligomeric membrane proteins together. They may also act as chaperones that facilitate folding and insertion of these proteins and ensure they adopt the correct topology.
Mutations in lipid hydrolases and other proteins cause disorders such as Niemann-Pick type C in which accumulation of one lipid species leads to coprecipitation of other hydrophobic molecules, which blocks the endolyosomal system.
Flippases move lipids from the outer leaflet of the membrane to the inner leaflet; floppases export them in the opposite direction. This creates an asymmetry critical for membrane function and facilitates vesicle budding. Sterols and sphingolipids exhibit diverse structures and physiological roles in eukaryotes.
They function in signaling, development, and may act together to regulate membrane trafficking and other cellular processes. Cell membranes have a complex organization, showing lateral heterogeneity on a range of scales. This can be controlled by changes in lipid composition, which generate distinct liquid-ordered and liquid-disordered phases. Atomistic and coarse-grained molecular dynamics simulations can accurately predict how lipid molecules behave in membranes, interact with proteins, and are trafficked within cells.
Lipid droplets are small organelles in which a phospholipid monolayer surrounds a lipid ester core. They are not just storage depots but contain various enzymes and may act as platforms for degradation of hydrophobic proteins. TOC Blurb: Lipid trafficking depends on lipid—lipid and lipid—protein interactions within membranes.
Trade-offs between bending energy, mixing entropy, and interactions between species may drive the sorting of lipids into transport vesicles. Glycosphingolipid Functions Clifford A. Frolov , Anna V. Distribution and Functions of Sterols and Sphingolipids J. Heberle , and Gerald W.
Current Issue February , 13 2. From the cover Auxin and Flower Development. Online ISSN:
2.8: Structure and Function - Lipids and Membranes
NCBI Bookshelf. Lipids are compounds that are insoluble in water but are soluble in organic solvents such as ether and chloroform. Lipids that are important to our discussion include fats and oils triglycerides or triacyglycerols , fatty acids, phospholipids, and cholesterol. Fats and oils are esters of glycerol and three fatty acids. They are important in the diet as energy sources and as sources of essential fatty acids and fat-soluble vitamins, which tend to associate with fats.
Hundreds of different lipid species are present in eukaryotic cell membranes. Some of them aggregate with specific membrane proteins to form specialized domains that concentrate and control cellular trafficking and signaling events. For both naked and enveloped viruses, viral entry, genome replication, and egress involve specific interactions with the membranes of a susceptible host cell. Their characterization has led to the discovery of lipid-active compounds as potential antiviral drugs. High-resolution mass spectrometry streamlines the shotgun analysis of total lipid extracts. It can distinguish and quantify isobaric lipid species and has potential to become the "gold standard" lipidomics methodology. Questions in modern lipid cell biology have been answered by membrane models such as SUVs, LUVs, and GUVs—closed lipid bilayers that are used to study effects of membrane permeability barriers and curvature.
The study of lipids has developed into a research field of increasing importance as their multiple biological roles in cell biology, physiology and pathology are becoming better understood. The Lipid Metabolites and Pathways Strategy LIPID MAPS consortium is actively involved in an integrated approach for the detection, quantitation and pathway reconstruction of lipids and related genes and proteins at a systems-biology level. A key component of this approach is a bioinformatics infrastructure involving a clearly defined classification of lipids, a state-of-the-art database system for molecular species and experimental data and a suite of user-friendly tools to assist lipidomics researchers.
Lipids are a diverse group of molecules that all share the characteristic that at least a portion of them is hydrophobic. Other, amphipathic lipids, such as glycerophospholipids and sphingolipids spontaneously organize themselves into lipid bilayers when placed in water. Interestingly, major parts of many lipids can be derived from acetyl-CoA. Figure 2. The most ubiquitous lipids in cells are the fatty acids.
Unlike other biomolecule groups, lipids are not defined by the presence of specific structural characteristics. Lipids are insoluble biomolecules, defined by an overall lack of polarity necessary for solubility in water-based solutions. In popular culture, fats are synonymous with lipids, giving lipids a negative role in diet and health. However, lipids play vital roles in many cellular processes including energy storage, structural support, protection, and communication. Common lipid groups include waxes, steroids, fats, and phospholipids.