Recombinant Dna Technology And Molecular Cloning Pdf

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Recombinant DNA technology and molecular cloning

Skip to content. Skip to navigation. In practice, the process often involves combining the DNA of different organisms. The process depends on the ability to cut and re-join DNA molecules at points which are identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using restriction enzymes also called restriction endonucleases and then inserted into other chromosomes or DNA molecules using enzymes called ligases. This describes the process of copying fragments of DNA which can then be used for many different purposes, such as creating GM crops, or finding a cure for disease. The other type is in vitro which is using the polymerase chain reaction PCR method to create copies of fragments of DNA.

If you're seeing this message, it means we're having trouble loading external resources on our website. To log in and use all the features of Khan Academy, please enable JavaScript in your browser. Donate Login Sign up Search for courses, skills, and videos. Introduction to genetic engineering. Overview: DNA cloning. Polymerase chain reaction PCR. Applications of DNA technologies.

Recombinant DNA

For all living organisms, the DNA deoxyribonucleic acid is the most important molecule. It carries genetic information that serves as the basis for development, growth, and reproduction among other important functions. Information contained in this molecule is first copied, through transcription, into mRNA before being translated to produce proteins. Here, scientists isolate a gene of interest from one organism and insert it into a vector thereby forming a recombinant DNA molecule. Large quantities of the gene of interest or products of the gene are produced when the bacteria carrying the vector multiplies to increase in numbers.


bacterial cells that arise from a single cell on a petridish are clones. Molecular biology has borrowed the term to mean a collection of molecules or cells all identical.


Recombinant DNA Techniques: Cloning and Manipulation of DNA

It is generated by transferring selected pieces of DNA from one organism to another. The vial shown in the photograph contains human insulin, one of the first therapeutic proteins that was genetically cloned. The drug is used to treat diabetes.

Recombinant DNA , molecules of DNA from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science , medicine , agriculture, and industry. Since the focus of all genetics is the gene , the fundamental goal of laboratory geneticists is to isolate, characterize, and manipulate genes. Although it is relatively easy to isolate a sample of DNA from a collection of cells , finding a specific gene within this DNA sample can be compared to finding a needle in a haystack. Consider the fact that each human cell contains approximately 2 metres 6 feet of DNA. Therefore, a small tissue sample will contain many kilometres of DNA.

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Overview: DNA cloning

Почти через двадцать лет, получив степень магистра математики в Университете Джонса Хопкинса и окончив аспирантуру по теории чисел со стипендией Массачусетского технологического института, она представила докторскую диссертацию- Криптографические методы, протоколы и алгоритмы ручного шифрования. По-видимому, ее работу прочел не только научный руководитель, потому что вскоре последовал телефонный звонок, а затем по почте ей доставили авиационный билет от АНБ.

 Его партнер опубликует ключ? - недоуменно переспросила Сьюзан. Стратмор кивнул: - Он разместит его в Интернете, напечатает в газетах, на рекламных щитах. Короче, он отдаст ключ публике. Глаза Сьюзан расширились.

Увидав королеву шифровалки Сьюзан Флетчер, Чатрукьян моментально отвел. Он боялся ее как огня. Ее мозги работали словно на совсем другом уровне. Она подавляла его своей красотой, и всякий раз, когда он оказывался рядом, язык у него заплетался.

 - Насколько я знаю Стратмора, это его дела. Готова спорить на любые деньги, что он. Чутье мне подсказывает.  - Второе, что никогда не ставилось под сомнение, - это чутье Мидж.

Recombinant DNA and genetic techniques